iPhage-F (Addition to Feed)

1. About New Line Group

We are a forward-thinking enterprise focused on revolutionizing bacteriophage solutions for the aquaculture sector. Our team comprises more than 30 Ph.D. and Master’s students, collaborating with esteemed institutions such as the Chinese Academy of Sciences, Jiangsu Province Academy of Agricultural Sciences and CTCbio, Korea. Leveraging the International Phage Research Center (IPRC), we continuously pioneer cutting-edge technologies.

Driven by our dedication to sustainable aquaculture, we strive to equip farmers with powerful tools to combat bacterial diseases effectively. iPhage-V emerges as the foremost bacteriophage solution for aquaculture, delivering unmatched advantages in raw materials, production processes, and efficacy.

Unmatched Raw Material Superiority:

Unprecedented Collection: We boast a vast collection of over 12,000 full bacteriophage genome sequences, representing a global repository of knowledge.
Extensive Preservation: Our extensive collection includes over 3,600 bacteriophage strains and over 43,000 epidemic pathogenic bacteria strains, providing a comprehensive foundation for research and development.
Pioneered Large-Scale Purification: Our cutting-edge production and purification system ensures exceptionally high purity of bacteriophage raw powder, exceeding 99%, resulting in a concentrated and effective product for aquaculture needs.

Unmatched Production Capacity:

State-of-the-Art Facilities: Our state-of-the-art production facilities stand as a testament to innovation, featuring not only 6 high-tech fermentation production lines but also 2 cutting-edge bacteriophage mixed production lines, exemplifying the forefront of contemporary manufacturing practices.
Massive Production Capacity: Boasting an impressive annual production capacity of 10,000 tons of bacteriophage products, our facility emerges as a beacon of efficiency, ready and equipped to effortlessly cater to the evolving needs and demands of the burgeoning aquaculture industry.

Unmatched Efficacy:

Precise Targeting:  iPhage is designed to specifically target and eliminate harmful Vibrio bacteria, effectively preventing various diseases in aquatic animals.
Safe and Sustainable:  Our bacteriophage-based solutions are environmentally friendly and do not pose a threat to water bodies or the ecological environment. Moreover, they do not contribute to antibiotic resistance.
Proven Results:  Extensive testing has demonstrated that iPhage-V is highly effective in controlling Vibrio bacteria in diverse aquaculture settings.

2. Bacteriophages, “antibiotics” from Nature

Bacteriophages, viruses that infect and reproduce within bacteria, possess the ability to specifically identify and break open bacterial cells through lysis. Their efficacy against multidrug-resistant bacteria, coupled with a reduced likelihood of inducing resistance, positions bacteriophages as a promising alternative to antibiotics. The enduring co evolutionary bond between bacteriophages and bacteria has equipped them with distinctive advantages in bacterial control.

Bacteriophage Action Mechanism

Recognition

The bacteriophages identifie and bind to a specific host bacterium.

Injection

It then injects its genetic material into the host.

Utilization

The host’s cellular machinery is hijacked to synthesize progeny phage proteins & genetic material.

Assembly

These components are assembled into new bacteriophages.

Lysis

The host cell is lysed, releasing the newly-assembled phages.

Re-infection

Newly formed bacteriophages infect other bacteria, continuing the cycle.

百噬特iPhage-F

噬菌体 取之自然的细菌解决方案 Phages: Bacterial solutions from nature.

耐高温 禽 猪 反刍 兔 宠物
High temperature resistant Poultry Swine Ruminants Rabbits Pets

什么是噬菌体？ What is a phage?

噬菌体是一种以细菌为宿主，选择性地攻击和杀死特定细菌，从而防控细菌性感染的病毒
A phage is a virus that uses bacteria as a host and selectively attacks and kills specific bacteria, thereby preventing and controlling bacterial infections

噬菌体是广泛存在于动物肠道、土壤、水体、植物中的自然生物
Phages are natural organisms that are widely found in animal intestines, soil, water, and plants

百噬特的稳定性 Stability of Iphage-V

85℃模拟制粒对噬菌体效价的影响  The Impact of Simulated Granulation at 85°C on the Potency of iPhage-F

不同酸碱度处理对噬菌体效价的影响  The Impact of Different pH Treatments on the Potency of iPhage-F

噬菌体对生物膜的作用 Phage Action on Biofilms

噬菌体能清除细菌在有生物活性或无生物活性的介质表面形成的生物被膜，将被膜中的细菌暴露在噬菌体或者抗生素的作用下。
Phages can remove biofilms formed by bacteria on the surface of active or inactive media, exposing the bacteria in the biofilm to the action of phages or antibiotics.

百噬特的部分靶向细菌及相关疾病 Selected Bacteria and Related Diseases Targeted by iPhage-F

菌属Genus菌名Bacteria相关疾病 Related Diseases肉鸡鸭
Duck & Chicken Broilers		蛋鸡种鸡 Chicken Layers Chicken Breeders猪Swine牛Cattle
大肠杆菌 Escherichia coli (E. coli)腹泻腹泻腹泻腹泻水肿病大肠杆菌病 Escherichia coli (E. coli)大肠杆菌病 Escherichia coli (E. coli)
沙门氏菌 Salmonella鸡沙门氏菌 Salmonella henselae鸡白痢沙门氏菌 Salmonella dysentery in chickens鼠伤寒沙门氏菌 salmonella typhimurium肠炎沙门氏菌 Salmonella enteritidis霍乱沙门氏菌 Salmonella cholerae都柏林沙门氏菌 Salmonella Dublin德比沙门氏菌 Salmonella Derby禽伤寒鸡白痢腹泻、副伤寒 Diarrhea, paratyphoid fever腹泻、副伤寒 Diarrhea, paratyphoid fever腹泻、败血症 Diarrhea, sepsis腹泻、败血症 Diarrhea, sepsis腹泻、肠炎 Diarrhea, enteritis
产气荚膜梭菌 Clostridium perfringensA型B型C型D型E型
Type A
Type B
Type C
Type D
Type E		腹泻、肠炎 Diarrhea, enteritis腹泻、肠炎 Diarrhea, enteritis腹泻、肠炎 Diarrhea, enteritis腹泻、肠炎 Diarrhea, enteritis腹泻、肠炎 Diarrhea, enteritis
葡萄球菌 staphylococcus金黄色葡萄球菌 Staphylococcus aureus关节肿大、表皮炎、 Enlarged joints, epidermolysis bullosa,乳腺炎、继发感染 Mastitis, secondary infection

噬菌体应用案例 iPhage-F Application Cases

(一)噬菌体对仔猪腹泻率的影响 (I) Effect of iPhage-F on diarrhea rate in piglets

试验设计:将腹泻仔猪单独挑出1g/头/天连续灌喂3天,或者1kg/T湿拌料连续饲喂3-5天,记录每天腹泻仔猪头数
Experimental design: select diarrheic piglets and administer 1 g/head/day by gavage for 3 consecutive days or 1 kg/t in wet feed for 3-5 consecutive days, and record daily the number of diarrheic piglets.

客户区域  Client Area饲喂方式  Feeding method腹泻仔猪（头）  Diarrhea piglets (head)成功率%
Success rate
第0天Day 1第1天 Day 2第2天 Day 3第3天 Day 4第4天 Day 5
四川Sichuan拌料Feed
灌服Gavage
山东Shandong灌服Gavage
安徽Anhui灌服Gavage
福建Fujian灌服Gavage

结论:在不同地域、猪场规模、管理水平和日龄情况下,噬菌体对腹泻仔猪均有较好的止泻效果。
Conclusion: Under different regions, pig farm sizes, management levels and ages, iPhage-F has a good anti-diarrheal effect on diarrheic piglets.

  1. 噬菌体对断奶仔猪生长性能和腹泻的影响 Effect of iPhage-F on growth performance and diarrhea in weaned piglets

试验地址:湖南某饲料企业养殖基地  Test site:A feed enterprise breeding base in Hunan province

试验设计:选择断奶仔猪192头，公母各半，初始体重9.76±0.17kg，随机分为4组，
Experimental design:192 weaned piglets, half male and half female, with an initial body weight of 9.76±0.17 kg were selected and randomly divided into four groups.

对照组饲喂基础日粮，试验组饲喂在基础日粮上添加不同剂量噬菌体日粮，试验期28天。
The control group was fed a basal diet and the experimental group was fed diets with different doses of iPhage-F added to the basal diet for 28 days.

项目Items对照组
Control Group		噬菌体添加量  iPhage-F addition
平均日增重，kg  Average daily weight gain, kg
平均日采食量，kg  Average daily feed intake, kg
料重比F:G  Material weight ratio F:G
腹泻评分1  Diarrhea Score 1

注:1根据形状及固体物含量对仔猪进行腹泻评分，分数越高腹泻越严重；
Notes:1 Piglets are scored for diarrhea based on shape and solids content, the higher the score the more severe the diarrhea;

*表示与对照组相比差异显著，**表示与对照组相比差异极显著。
* indicates a significant difference compared to the control group and ** indicates a highly significant difference compared to the control group.

结论:在断奶仔猪饲料中添加噬菌体产品，可以改善腹泻评分及料比。
Conclusion:Addition of iPhage-F to weaned piglet feed improves diarrhea scores and feed ratios.

  1. 噬菌体对肉鸡生长性能的影响 Effect of iPhage-F on growth performance of broiler chickens
    试验地址:某外资肉鸡养殖企业   Test site: A foreign-funded broiler enterprise

试验设计:4栋平养鸡舍，随机分为两组，对照组饲喂基础日粮，试验组日粮中全程添加噬菌体产品(前期200 g/t，后期150 g/t)
Experimental design:4 flat chicken houses were randomly divided into two groups. The control group was fed a basal diet, and the experimental group was supplemented with phage products (200 g/t in the early stage and 150 g/t in the later stage) throughout the diet.

组别Groups出栏日龄  Days to Market出栏体重kg  Market WeightADG（g）成活率（%）  Survival rate FCR欧指EBI
对照组Control Group
噬菌体组  iPhage-F Group

结论:肉鸡饲料中添加噬菌体产品，可以有效提高成活率、降低料比、改善欧指。
Conclusion:The addition of iPhage-F to broiler feed can effectively increase the survival rate, reduce the feed ratio, and improve the European Broiler Index (EBI).

  1. 噬菌体对种鸡沙门氏菌阳性率的影响 Effect of iPhage-F on Salmonella positivity in breeder chickens

试验地点:山西某大型养殖公司100万羽种鸡场  Test site: 1,000,000-bird breeder farm of a large-scale breeding company in Shanxi, China

试验设计:分别选择6栋种鸡舍，每栋鸡舍10000羽，试验前全血平板凝集试验测定沙门
Experimental design:6 chicken breeder houses were selected, each with 10,000 birds, and the whole blood plate agglutination test was used to determine Salmonella spp. before the test.

氏菌阳性率，选择沙门氏菌阳性率高的三栋为试验组，沙门氏菌阳性率低的三栋为对照组，对照组饲喂基础日粮，试验组在基础日粮中添加噬菌体产品200g/吨，试验期2个月。

The three houses with high Salmonella positivity were selected as the test group, and the three buildings with low Salmonella positivity were selected as control group. The control group was fed the basal diet, and the test group was added 200g/ton of iPhage-F to the basal diet for 2 months.

组别Group栋舍House #沙门氏菌阳性率  Salmonella positivity rate
对照组Control Group2#
3#
4#
平均Average
噬菌体组  iPhage-F1#
7#
8#
平均Average

结论:种鸡饲料中添加噬菌体产品能够有效控制和降低种鸡沙门氏菌感染率。
Conclusion:The addition of iPhage-F to breeder feed can effectively control and reduce the infection rate of Salmonella in breeders.

  1. 噬菌体对蛋鸡生产性能和肠道细菌的影响 Effect of iPhage-F on performance and intestinal bacteria of laying hens

试验地点:湖北某蛋鸡养殖场  Location:An egg farm in Hubei Province, China

试验设计:36周龄海兰褐蛋鸡288只，随机分为三组，每组6个重复，对照组饲喂基础日粮，试验组饲喂基础日粮添加噬菌体日粮，试验期6周。Experimental design:288 Hyland Brown laying hens at 36 weeks of age were randomly divided into three groups with six replicates in each group, and the control group was fed a basal diet, and the test group was fed the basal diet plus iPhage-F diet for 6 weeks.

项目Items对照组Control Group产蛋率%  Egg production %
噬菌体 100g/t  iPhage-F 100g/t噬菌体 200g/t  iPhage-F 200g/t

结论:蛋鸡饲料中添加噬菌体产品能够有效提高蛋鸡产蛋率，减少肠道大肠杆菌和沙门氏菌数量。
Conclusion:Addition of iPhage-F to laying hen feed can effectively increase egg production rate and reduce the number of intestinal Escherichia coli and Salmonella in laying hens.

噬菌体与抗生素协同杀菌 iPhage-F and antibiotics synergize to kill bacteria

噬菌体与抗生素的杀菌机制不同The bactericidal mechanisms of phages and antibiotics are different.  

Phage-antibiotic synergistic effect

Antibiotic Phage Biofilm Bacteria

噬菌体可以杀灭抗生素耐药细菌  Phages can kill antibiotic-resistant bacteria.

噬菌体-抗生素协同效应 抗生素 噬菌体 生物膜 细菌
Phage-antibiotic synergistic effect Antibiotics Phages Biofilm Bacteria

百噬特的优势 Advantages of iPhage-F

噬菌体只对靶向细菌有作用，不影响肠道的有益菌群  Phages only act on targeted bacteria and do not affect the beneficial flora of the gut

安全性高，无毒副作用  Highly safe, no toxic or side effects

没有停药期  No withdrawal period

取之自然的细菌解决方案 Bacterial solutions from nature

靶向细菌  Targeting Bacteria沙门氏菌:鸡白痢、伤寒、肠炎、鼠伤寒、德比、都柏林、霍乱沙门氏菌……
Salmonella:Fowl dysentery, typhoid, enteritis, murine typhoid, Derby, Dublin, cholera Salmonella ……大肠杆菌:F4(K88)、F5(K99)、F6(987P)、F18、F41、O78、O39、O86、
E. coli:F4(K88), F5(K99), F6(987P), F18, F41, O78, O39, O86,O157、O88、O2、O45、O53、O145……
o157, o88, o2, o45, o53, o145 ……产气英膜梭菌:A型、B型、C型、D型、E型   Clostridium perfringens: type A, B, C, D, E金黄色葡萄球菌   Staphylococcus aureus
推荐剂量g/t  Recommended dose g/t猪Swine 乳仔猪300-500   Suckling piglets 300-500中大猪100-200  Growing pigs 100-200母 猪200-300   Sows 200-300禽Poultry 肉鸡100-200  Chicken Broilers 100-200蛋鸡100-200   Chicken Layers 100-200种鸡100-200   Chicken Breeders 100-200其他动物  Other animals兔 300-500   Rabbit 300-500反刍动物 300-500   Ruminants 300-500宠 物 300-500   Pets 300-500
主要成分  base复合噬菌体、枯草芽孢杆菌(≥10 CFU/g)、载体
Complex phages, Bacillus subtilis (≥10 CFU/g), carrier
类 型预混微粉制剂  Pre-mixed micropowder formulations
包装规格  Packaging specification20 kg/袋  20 kg/bag
用 途预防和控制各种靶向细菌造成的肠道及组织器官感染  Prevention and control of intestinal and tissue organ infections caused by various targeted bacteria
贮存条件  storage condition阴凉、干燥、通风、避光处保存  Store in a cool, dry, ventilated place, protected from light
保 质 期  Warranty12个月  12 months
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