1. About New Line Group

We are a forward-thinking enterprise focused on revolutionizing bacteriophage solutions for the aquaculture sector. Our team comprises more than 30 Ph.D. and Master’s students, collaborating with esteemed institutions such as the Chinese Academy of Sciences, Jiangsu Province Academy of Agricultural Sciences and CTCbio, Korea. Leveraging the International Phage Research Center (IPRC), we continuously pioneer cutting-edge technologies.

Driven by our dedication to sustainable aquaculture, we strive to equip farmers with powerful tools to combat bacterial diseases effectively. iPhage-V emerges as the foremost bacteriophage solution for aquaculture, delivering unmatched advantages in raw materials, production processes, and efficacy.

Unmatched Raw Material Superiority:

Unprecedented Collection: We boast a vast collection of over 12,000 full bacteriophage genome sequences, representing a global repository of knowledge.
Extensive Preservation: Our extensive collection includes over 3,600 bacteriophage strains and over 43,000 epidemic pathogenic bacteria strains, providing a comprehensive foundation for research and development.
Pioneered Large-Scale Purification: Our cutting-edge production and purification system ensures exceptionally high purity of bacteriophage raw powder, exceeding 99%, resulting in a concentrated and effective product for aquaculture needs.

Unmatched Production Capacity:

State-of-the-Art Facilities: Our state-of-the-art production facilities stand as a testament to innovation, featuring not only 6 high-tech fermentation production lines but also 2 cutting-edge bacteriophage mixed production lines, exemplifying the forefront of contemporary manufacturing practices.
Massive Production Capacity: Boasting an impressive annual production capacity of 10,000 tons of bacteriophage products, our facility emerges as a beacon of efficiency, ready and equipped to effortlessly cater to the evolving needs and demands of the burgeoning aquaculture industry.

Unmatched Efficacy:

Precise Targeting:  iPhage is designed to specifically target and eliminate harmful Vibrio bacteria, effectively preventing various diseases in aquatic animals.
Safe and Sustainable:  Our bacteriophage-based solutions are environmentally friendly and do not pose a threat to water bodies or the ecological environment. Moreover, they do not contribute to antibiotic resistance.
Proven Results:  Extensive testing has demonstrated that iPhage-V is highly effective in controlling Vibrio bacteria in diverse aquaculture settings.

2. Impact of Vibrio on Aquatic Animals

Prevalent Aquatic Animal Vibrio Diseases

Vibriosis stands as one of the prevalent bacterial diseases impacting shrimp, fish, and shellfish across aquatic environments. Vibrio, functioning as an opportunistic pathogen, infiltrates aquatic animals via various entry points such as the mouth, gills, and skin. This can result in primary or secondary infections, particularly during environmental deteriorations like rainy weather or significant diurnal temperature variations. Additionally, decreased resistance in aquatic animals or co-infections with other bacterial or viral diseases can exacerbate the susceptibility to Vibrio infections.

Click for Fish Diseases and their Causative Pathogens
Fish Diseases and their Causative Pathogens
Pathogen——–> Disease
Vibrio harveyi – Scale loss & muscle necrosis vasculitis
Vibrio harveyi – Eye lesions
Vibrio harveyi– Infectious necrotizing enteritis
Vibrio parahaemolyticus– Tail rot disease
Vibrio anguillarum– Red worm disease of eel
Vibrio anguillarum– Red spot disease
Vibrio anguillarum– Ulcer syndrome
Vibrio vulnificus– Warm-water vibriosis
Click for Shellfish Diseases & their Causative Pathogens
Shellfish Diseases and their Causative Pathogens
Pathogen——–> Disease
Vibrio harveyi, Vibrio parahaemolyticus,etc.– Abalone muscle atrophy syndrome
Vibrio alginolyticus, etcAcute detachment of juvenile abalone
Vibrio harveyi– Pustulosis of Haliotis discus hannai Ino
Vibrio harveyi– Swollen-mouth disease of Babylonia areolata
Click for Shrimp Diseases & their Causative Pathogens
Shrimp Diseases and their Causative Pathogens
Pathogen——–> Disease
Vibrio parahaemolyticus– Acute hepatopancreatic necrosis syndrome of shrimp
Vibrio harveyi – Luminous vibriosis of shrimp
Vibrio harveyi, etc– Gill rot disease of shrimp
Vibrio parahaemolyticus, Vibrio anguillarum, Vibrio alginolyticus, etc.– Red leg disease of shrimp
Vibrio anguillarum, Vibrio vulnificus, Vibrio alginolyticus, Vibrio parahaemolyticus, etc.– Vibriosis of shrimp larvae
Click for Crab Diseases & their Causative Pathogens
Crab Diseases and their Causative Pathogens
Pathogens——–> Disease
Vibrio parahaemolyticus, Vibrio anguillarum, Vibrio alginolyticus, etc. – Crab Vibriosis

3. Difficulties in Vibriosis Prevention and Control:

01

Wide Temperature Adaptability

Vibrio bacteria exhibit resilience across a broad temperature spectrum, thriving in environments ranging from 10℃ to 35℃, with optimal conditions typically observed around 25℃. This adaptability enables vibriosis to pose a persistent threat to aquaculture year round.

02

Broad pH Tolerance

Vibrio bacteria can cause outbreaks in a wide pH range (6-10). This tolerance signifies their ability to survive and multiply in most aquatic environments, making them a persistent challenge.

03

Prone to Recurrence

Vibrio bacteria reside in large numbers within pond sediment. If ponds aren’t thoroughly cleaned after an aquaculture cycle, vibriosis is highly likely to recur in the subsequent cycle.Vibrio bacteria reside in large numbers within pond sediment.

04

Antibiotic Resistance

Excessive use of antibiotics and chemical disinfectants in quaculture leads to water pollution, disruption of microbial balance, and the proliferation of antibiotic-resistant strains and residues. Consequently, this poses a dual threat to farming efficiency and food safety.

4. Tiny but Mighty Bacteriophages

Bacteriophages, viruses that infect and reproduce within bacteria, possess the ability to specifically identify and break open bacterial cells through lysis. Their efficacy against multidrug-resistant bacteria, coupled with a reduced likelihood of inducing resistance, positions bacteriophages as a promising alternative to antibiotics. The enduring co evolutionary bond between bacteriophages and bacteria has equipped them with distinctive advantages in bacterial control.

Bacteriophage Action Mechanism

Recognition

The bacteriophages identifie and bind to a specific host bacterium.

Injection

They then inject their genetic material into the host.

Utilization

The host’s cellular machinery is hijacked to synthesize progeny phage proteins & genetic material.

Assembly

These components are assembled into new bacteriophages.

Lysis

The host cell is lysed, releasing the newly-assembled phages.

Re-infection

Newly formed bacteriophages infect other bacteria, continuing the cycle.

5. Unrivaled Advantages of Applying iPhage-V

iPhage-V: Pioneering Safe and Sustainable Aquaculture

By harnessing the innate capabilities of natural bacteriophages, iPhage-V propels us towards a future of sustainable aquaculture. It ensures a secure, nourishing, and plentiful food source for future generations. iPhage-V stands as the cornerstone for unlocking the complete potential of your aquaculture endeavors, fostering resilience, and cultivating a sustainable global food ecosystem.

No drug residues

Phages, acting as bacterial predators, leave no residues compared to drugs. They utilize bacterial machinery for replication, eliminating targets without introducing foreign chemicals.

Natural & easily accessible

Phages, the Earth’s most prevalent life form, exist naturally in our environment, including animal intestines, water bodies, and soil. This ubiquity highlights their natural origin and inherent compatibility with the ecosystem. 

Variety of administration modes

iPhage-V’s innovative powder form allows for convenient mixing into animal feed or direct application onto aquaculture water surfaces, simplifying administration.

Continuously Effective

Infecting and replicating within bacteria, phages can selectively identify and lyse them. This ability, alongside their effectiveness against multidrug-resistant bacteria and low potential for inducing resistance, positions phages as a promising antibiotic alternative. Their longstanding co-evolutionary relationship with bacteria has conferred unique advantages on bacteriophages for controlling bacterial populations.

Less susceptible to developing drug resistance

Co-evolution with bacteria inherently minimizes resistance development. iPhage’s cocktailed-phage formulation further strengthens this advantage, since a single bacterium is highly unlikely to develop resistance to all the phages in iPhage simultaneously.

Strongly specific

Precise Targeting: Unlike antibiotics, iPhage exhibits remarkable specificity. It recognizes and targets only harmful vibrios, leaving the beneficial gut bacteria and the surrounding environment completely unharmed. This targeted approach promotes animal health while minimizing disruption to the delicate ecological balance.

Exponentially Bactericidal

Phage proliferation grows exponentially, rapidly reaching high concentrations even with minimal doses, which minimizes the need for frequent administrations. This streamlined approach not only enhances bacterial population management but also optimizes disease control strategies, presenting a more efficient and targeted solution to combat infections.

6. Selected Laboratory Isolates of Vibrio

Selected Laboratory Isolates of Vibrio

7. Nemesis of Drug-Resistant Bacteria: iPhage-V

Targeted Pathogenic Bacteriatarget, aim, arrow-893324.jpg

  • Vibrio parahaemolyticus
  • Vibrio harveyi
  • Vibrio alginolyticus
  • Vibrio anguillarum
  • Vibrio vulnificus
  • Vibrio cholerae
  • Vibrio campbellii

Product Information of iPhage-V

Application Scenarios:

Hatcheries, elevated (high-density) ponds, industrialized farms, small greenhouse tunnels, earthen ponds.

Purposes

Prevent and control diseases caused by vibrios in the aquaculture process of shrimp, fish, sea cucumbers, crabs, and shellfish.

Physical Characteristics:

Appearance: White to light brown powder formulated with glucose as a carrier.
Quality: Free of mold, deterioration, caking, and foreign odor.
Moisture Content: ≤ 12%.
Heavy Metal Limits:Lead (Pb): ≤ 40 mg/kg; Total Arsenic (As): ≤ 10 mg/kg.
Microbiological Specifications: Total Mold Count: ≤ 10,000 CFU/g; Salmonella: Must not be detected in 1 g

Shelf Life, Storage and Handling:

Shelf life: 12 months.
Store this product in a cool, dry place away from direct sunlight and ensure it remains sealed when not in use. To maintain product efficacy, avoid mixing or co-applying this product with disinfectants, acidifiers, or alcohol during use.

Recommended Dosage of iPhage-V

General Use:

Evenly sprinkle 100g of iPhage-V mixed with water over 5-10 mu (1 Mu = 666.66 m2) of water surface, for 3-5 consecutive days, with a 15-day interval between each use. Increase dosage accordingly during high-temperature seasons.

Use as a Feed Additive

Mix with feed for feeding, using 100g of IPhage-V mixed with 100-200 kg of feed, for 3-5 consecutive days, with a 15-day interval between each use. Increase dosage accordingly during high-temperature seasons.
Note: Double the dosage in case of disease outbreak.

8. Efficacy Cases of iPhage-V in Treating Vibriosis

iPhage-V Treating Vibriosis Case 1

Location:

Lianyungang, Jiangsu Province, China

Mode:

High-density (elevated) ponds

Treatment Targets:

Whiteleg shrimp (Litopenaeus vannamei).

Treatment Method:

Diluted IPhage-V with water, 0.3g per cubic meter of water, evenly spread over the entire pond, used for 3 consecutive days

Conclusion:

Successfully eradicated severe Vibriosis, leading to natural recovery within 7 days, resulting in highly satisfied customers.

iPhage-V Treating Vibriosis Case 2

Location:

Yancheng, Jiangsu Province, China

Mode:

Small sheds

Treatment Targets:

Whiteleg shrimp (Litopenaeus vannamei).

Conclusion:

Successfully eradicated severe Vibriosis, leading to natural recovery within 7 days, resulting in highly satisfied customers.

Treatment Method:

Water Spray Treatment:
• Dilute iPhage-V with water at a ratio of 0.25g per cubic meter (equivalent to 100g per shed).
• Sprinkle the solution evenly across the entire pond.
• Repeat this process once daily for a period of 5 consecutive days.
Spontaneous Feed Treatment:
• Concurrently with the water spray treatment, incorporate iPhage-V into the feed, using 100g of iPhage-V for every 100kg of feed.
• Distribute the treated feed twice daily for 5 consecutive days, or up to 7 consecutive days in severe cases.

iPhage-V Treating Vibriosis Case 3

Location:

Yancheng, Jiangsu Province, China

Mode:

Outdoor ponds

Treatment Targets:

Whiteleg shrimp (Litopenaeus vannamei).

Treatment Method:

Treatment Regimen：
• Use a diluted solution of iPhage-V for treatment, applying 0.25g per cubic meter of water.
• Sprinkle the solution evenly throughout the entire pond.
• Continue this process daily for a period of 5 consecutive days.

Conclusion:

The treatment effectively managed highly pathogenic Vibrios, thereby enhancing the survival rate of shrimp, and increasing aquaculture profitability.

iPhage-V Treating Vibriosis Case 4

An iPhage-V Applicaiton Case of Treating Vibriosis in a Hatchery

Location:

A shrimp hatchery in Jiangsu Province, China

Treatment Details:

On December 20, 2023, a high incidence of Vibrio parahaemolyticus (VP) was detected in Pond No. 8 during a fluorescence quantitative PCR inspection. To address this, IPhage-V was administered at a concentration of 3ppm mixed with feed. This treated feed was given once daily for a total of three meals over three consecutive days. By the fourth day, the presence of VP had decreased to undetectable levels.

Conclusion:

iPhage-V demonstrated a significant preventive and therapeutic effect against Vibrio species, specifically yellow and green Vibrio (Vibrio parahaemolyticus), in both the water and within the shrimp themselves. This led to an overall improvement in the health of the shrimp population.

iPhage-V Treating Vibriosis Case 5

An iPhage-V Applicaiton Case of Treating Vibriosis in a Shrimp Nursery

Location:

A shrimp nursery in Jiangsu Province, China

Treatment Details:

In Pond No. 4, a severe infection of Vibrio harveyi and Vibrio parahaemolyticus was identified through a fluorescence quantitative PCR test. To combat this, IPhage-V was administered at a concentration of 3ppm. This was mixed with shrimp flakes and incorporated into the feed water, which was then sprinkled across the pond. This treatment was applied once daily for a consecutive period of five days. By the sixth day, the pathogens were no longer detectable, and the shrimp seedlings exhibited normal health in the subsequent period.

Conclusion:

iPhage-V demonstrated significant efficacy in controlling both Vibrio harveyi and Vibrio parahaemolyticus in the water and within the shrimp, contributing to a healthier shrimp population in the nursery.

8. Advantage Comparison of iPhage-V & Other “Phage-based” Products

AspectsiPhage-VOther “Phage-based” Products
Thermal ToleranceiPhage-V can endure up to 90°C.Others, in liquid form, are usually not resistant to high temperatures, may require refrigeration.
Bacterial CoverageiPhage-V covers over 85% of pathogenic bacterial strains.Others have been found out only to cover between 20% and 30% of pathogenic bacterial strains.
Shelf LifeOur specially formulated, powdered iPhage boasts an extended shelf life of at least 12 months. Others, in liquid,may have a shorter shelf life due to their susceptibility to bacterial contamination, typically lasting only 40 to 80 days after opening.
Targeted Treatment iPhage treatment effectively addresses a range of high-impact diseases, including high pathogenic Vibrio, EMS, and Hepatopancreatic Necrosis Syndrome. Long-term use can also help prevent these diseases and reduce high mortality rates.Our commitment to maintaining the industry’s most comprehensive bacterial library and phage banks fuels continuous innovation, ensuring we stay ahead of evolving threats.

9. Synergistic Effects of iPhage-V with Other Products

iPhage-V and Antibiotics:

iPhage-V significantly enhances the effect of antibiotics, effectively lowering their MIC (Minimum Inhibitory Concentration), thereby reducing antibiotic resistance.

iPhage-V & Probiotics / Microecological Preparations:

iPhage-V works synergistically with probiotics and microecological preparations. Phages iPhage-V effectively eliminate pathogenic Vibrios, aiding probiotics in colonization, accelerating microbial balance, enhancing antibacterial efficiency, promoting digestion and absorption, improving feed conversion rate, and boosting production performance.

iPhage-V & Trace Elements / Vitamins

iPhage-V enhances the effect of trace elements and vitamins, improving antioxidant levels and promoting immune regulation. The bacterial body can mitigate inflammation by adsorbing LPS, altering the expression of inflammation genes, inhibiting phagocytosis, and thus reducing oxidative stress.

iPhage-V and Plant Extracts

iPhage-V works synergistically with plant extracts, effectively enhancing antibacterial efficiency, improving the stress resistance of aquatic animals, boosting antioxidant levels, and regulating immune balance.

iPhage-V & Antimicrobial Peptides:

iPhage-V accelerates the destruction of biofilms, improves bactericidal efficiency, significantly reduces bacterial resistance, and promotes immune regulation.

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