Scientific Reports 科学报告 volume 14, Article number: 2657 (2024) , 文章编号：2657 (2024)
Abstract 摘要
Bacteriophage therapy is one potential strategy to treat antimicrobial resistant or persistent bacterial infections, and the year 2021 marked the centennial of Felix d’Hérelle’s first publication on the clinical applications of phages. At the Center for Phage Biology & Therapy at Yale University, a preparatory modular approach has been established to offer safe and potent phages for single-patient investigational new drug applications while recognizing the time constraints imposed by infection(s). This study provides a practical walkthrough of the pipeline with an Autographiviridae phage targeting Pseudomonas aeruginosa (phage vB_PaeA_SB, abbreviated to ΦSB). Notably, a thorough phage characterization and the evolutionary selection pressure exerted on bacteria by phages, analogous to antibiotics, are incorporated into the pipeline.
噬菌体疗法是治疗抗菌药耐药性或顽固性细菌感染的一种潜在策略，2021 年是费利克斯-德-赫雷勒（Felix d’Hérelle）首次发表噬菌体临床应用论文一百周年。耶鲁大学噬菌体生物学与治疗中心（Center for Phage Biology & Therapy at Yale University）建立了一种预备性模块方法，为单个病人的新药研究应用提供安全、有效的噬菌体，同时考虑到感染所带来的时间限制。本研究利用针对铜绿假单胞菌的 Autographiviridae 噬菌体（vB_PaeA_SB 噬菌体，缩写为 ΦSB）对该方法进行了实际演练。值得注意的是，噬菌体的全面特征描述和噬菌体对细菌施加的进化选择压力（类似于抗生素）都被纳入了研究流程。
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